Education
University of North Carolina at Charlotte, Charlotte, NC, B.A., Magna Cum Laude, Major: Biology , Minor: Chemistry, 1992.
Wake Forest University, Department of Microbiology and Immunology, Winston-Salem, NC, Ph.D. (Microbiology and Immunology) , 1998.
Duke University Medical Center, Department of Radiation Oncology, Durham, NC, Research Associate, 1998 - 2003.
Employment
Lab Assistant, Department of Civil Engineering, (Pseudomonas denitrificans propagation), University of North Carolina at Charlotte, 1990 - 1991.
Research Assistant, Department of Biology, University of North Carolina at Charlotte, 1990 - 1991.
Research Assistant Professor, Department of Obstetrics and Gynecology, Duke University, 2003 - present.
Teaching Experience
Bowman Gray School of Medicine
- Medical Microbiology Laboratory Instructor, 1994 - 1997.
- Medical Microbiology Tutor, 1997.
- Advanced Topics in Microbiology and Immunology, 1994 - 1997.
- Fundamentals of Virology - ad hoc Lecturer, 1997.
Human Genome Project
- American Society of Human Genetics Volunteer, Mentorship Network, 2001 - present.
Honors/Awards
Beta Beta Beta Biological Honor Society, 1989
Golden Key National Honor Society, 1990
Honor Society of Phi Kappa Phi, 1990
Omicron Delta Kappa Leadership Honor Society, 1991
North Carolina Academy of Science Award, 1991. Sigma Xi
Wake Forest University Graduate Fellowship, 1993 - 1994 and 1996 - 1997.
Predoctoral Fellowship, NIH Training Grant, 1994 - 1996 and 1997 - 1998.
Invention Disclosure 98-43: Method to design attenuated vaccine strains of an RNA virus. S. K. Murphy and G. D. Parks, Inventors, Wake Forest University School of Medicine, 1998.
Patent Application
Diagnostic test for the callipyge phenotype. Freking, B. A., Smith, T. P. L., Leymaster, K. A., Murphy SK, and R. L. Jirtle, Inventors, USDA MARC, Clay Center, Nebraska, and Duke University, 2002.
Grants and Funding
Yarbrough Undergraduate Research Grant, "Gene Transfer in a Nonculturable Bacterium," 1990.
Postdoctoral Fellowship, National Research Service Award, NCI, "Imprinted PEG3 Domain at 19q13.4 and Carcinogenesis," 2001-2003
Academic/Professional Service
Duke University Family Issues Task Force Postdoctoral Representative, 2002 - 2003.
Duke University Postdoctoral Association Mentorship Program: Founder and Coordinator, 2002 - 2003.
Duke University Postdoctoral Association: Chair, Advocacy Committee, 2001 - 2002.
Human Genome Project, American Society of Human Genetics, Volunteer, Mentorship Network, 2002 - present.
Professional Societies
American Association for the Advancement of Science, 1988
American Society of Microbiology, 1988
American Society for Virology, 1993
American Society of Human Genetics, 2000
Duke University Postdoctoral Association, 2001
Seminars
Murphy SK, and Parks, G.D. Sequence-Dependent and -Independent Features of the Paramyxovirus Promoter that Govern Viral RNA Replication. Triangle Virology Spring Symposium, North Carolina Biotechnology Center, 1998.
Murphy SK. An Introduction to Profile Hidden Markov Models. Duke Bioinformatics Shared Resource Seminar Series, Duke University Medical Center, 2000.
Genbank Submissions
Smith,T. P. L. and Murphy SK. Identification of the single base change causing the callipyge muscle hypertrophy phenotype, the only known example of polar overdominance in mammals. Submitted July 26, 2002. Accession No. AF533009.
Publications
1. Murphy SK, and Oliver, J.D. Effects of Temperature Abuse on Survival of Vibrio vulnificus in Oysters. Appl. Environ. Microbiol. 58: 2771-2775, 1992.
2. Murphy SK, and Parks, G.D. Genome Nucleotide Lengths Divisible by Six are not Essential but Enhance Replication of Defective Interfering RNAs of the Paramyxovirus Simian Virus 5. Virology 232: 145-157, 1997. [PDF]
3. Murphy SK, Ito, Y., and Parks, G.D. A Functional Antigenomic Promoter for the Paramyxovirus Simian Virus 5 Requires Proper Spacing between an Essential Internal Segment and the 3' Terminus. J. Virol. 72:10-19, 1998. [PDF]
4. Murphy SK, and Parks, G.D. RNA Replication for the Paramyxovirus Simian Virus 5 Requires an Internal Repeated (CGNNNN) Sequence Motif. J. Virol. 73:805-809, 1999. [PDF]
5. Murphy SK, and Jirtle, R.L. Imprinted genes as potential genetic and epigenetic toxicologic targets. Environ. Health Perspect. 108 (Suppl. 1) : 5-11, 2000. [PDF]
6. Wylie, A.A., Murphy SK, Orton, T.C., and Jirtle, R.L. Novel imprinted DLK1/GTL2 domain on human chromosome 14 contains motifs that mimic those implicated in IGF2/H19 regulation. Genome Res. 10: 1711-1718, 2000. [PDF]
7. Murphy SK, Wylie, A.A., and Jirtle, R.L. Imprinting of PEG3,the human homolog of a gene involved in nurturing behavior in mice. Genomics 71: 110-117, 2000. [Journal Cover][PDF]
8. Evans, H.K., Wylie, A.A., Murphy, S.K, and Jirtle, R.L. The neuronatin gene resides in a "micro-imprinted" domain on human chromosome 20q11.2. Genomics 77: 99-104, 2001. [PDF]
9. Keller, M.A., Murphy SK, and Parks, G.D. RNA replication from the simian virus 5 antigenomic promoter requires three sequence-dependent elements separated by sequence-independent spacer regions. J. Virol. 75: 3993-3998, 2001. [PDF]
10. Murphy SK, and Jirtle, R.L. Non-Genotoxic Causes of Cancer. In: Cancer Handbook, (Gooderham, N., ed.), pp. 317-333, Nature Publishing Group Reference, London, UK, 2001.
11. Li, T., Vu, T.H., Lee, K.O., Yang Y., Nguyen, C.V., Bui, H.Q., Zeng, Z.L., Nguyen, B.T., Hu, J.F., Murphy SK,Jirtle, R.L., and Hoffman, A.R. An imprinted PEG1/MEST antis ense expressed predominantly in human testis and in mature spermatozoa. J. Biol. Chem. 277: 13518-27, 2002. [PDF]
12. Freking, B.A., Murphy SK, Wylie, A.A., Rhodes, S.J., Keele, J.W., Leymaster, K.A., Jirtle, R.L., and Smith, T.P. Identification of the single base change causing the callipyge muscle hypertrophy phenotype, the only known example of polar overdominance in mammals. Genome Res. 12: 1496-1506, 2002. [Journal Cover][PDF]
13. Dietz, L.G., Wylie, A.A., Rauen, K.A., Murphy SK, Jirtle, R.L., and Cotter, P.D. Exclusion of maternal uniparental disomy of chromosome 14 in patients referred for Prader-Willi syndrome using a multiplex methylation polymerase chain reaction assay. J. Med. Genet. 40: e46, 2003.
14. Murphy SK, and Jirtle, R.L. Imprinting evolution and the price of silence. BioEssays 25: 577-588, 2003. [PDF]
15. Murphy SK, Wylie, A.A., Coveler, K.J., Cotter, P.D., Papenhausen, P.R., Sutton, V.R., Shaffer, L.G., and Jirtle, R.L. Epigenetic detection of human chromosome 14 uniparental disomy Hum. Mutat. 22: 92-97, 2003. [PDF]
16. Weidman, J.R., Murphy SK, Nolan, C.M., Dietrich, F.S., and Jirtle, R.L. Phylogenetic footprint analysis of IGF2 in extant mammals. Genome Res. 14: 1726-1732, 2004. [Journal Cover] [PDF]
17. Wang W., Huper G., Guo Y., Murphy SK, Olson J.A., Jr., and Marks J.R. Analysis of methylation-sensitive transcriptome identifies GADD45a as a frequently methylated gene in breast cancer. Oncogene. 24: 2705-2714, 2005.
18. Murphy SK, Freking, B.A., Smith, T.P.L., Leymaster, K., Nolan, C.M., Wylie, A.A., Evans, H.K., and Jirtle RL. Abnormal postnatal maintenance of elevated DLK1 transcript levels in callipyge sheep. Mamm. Genome 16: 171-183, 2005. [PDF]
19. Murphy SK, Nolan, C.M., Huang, Z., Kucera, K.S., Freking, B.A., Smith, T.P.L., Leymaster, K.A., Weidman, J.R., and Jirtle, R.L. Callipyge mutation affects gene expression in cis: a potential role for chromatin structure. Genome Res. 16: 340-346, 2006. [PDF]
20. Berchuck, A., Iversen, E. S., Lancaster, J. M., Pittman, J., Luo, J., Lee, P., Murphy SK, Dressman, H. K., Febbo, P. G., West, M., Nevins, J. R., and Marks, J. R. Patterns of gene expression that characterize long-term survival in advanced stage serous ovarian cancers. Clin. Cancer Res. 11: 3686-3696, 2005.
21. Spillman, M.A., Schildkraut, J. M., Halabi, S., Moorman, P., Calingaert, B., Bentley, R. C., Marks, J. R., Murphy, S., and A. Berchuck. Transforming Growth Factor § Receptor 1 polyalanine repeat polymorphism does not increase ovarian cancer risk. Gynecol. Oncol. 97: 543-549, 2005.
22. Murphy SK, Huang, Z., Wen, Y., Spillman, M.A., Whitaker, R.S., Simel, L.R., Nichols, T.D., Marks, J.R., and Berchuck, A. Frequent IGF2/H19 domain epigenetic alterations and elevated IGF2 expression in epithelial ovarian cancer. Mol. Cancer Res. 4: 283-292, 2006.
23. Huang, Z., Wen, Y., Shandilya, R., Marks, J.R., Berchuck, A., and Murphy SK High throughput detection of M6P/IGF2R intronic hypermethylation and LOH in ovarian cancer. Nucleic Acids Res. 34: 555-563, 2006.
24. Risinger, J. I., Custer, M., Pack, S., Loukinov, D., Maxwell, G. L., Chandramouli, G., Aprelikova, O., Litzi, T., Murphy SK, Berchuck, A., Lobanenkov, V., and J.C. Barrett. Expression analysis of cancer testis antigens in uterine cancers details a high incidence of BORIS expression. Clin Cancer Res. 13: 1713-1719, 2007.
25. Schildkraut JM, Murphy SK, Palmieri RT, Iversen E, Moorman PG, Huang Z, Halabi S, Calingaert B, Gusberg A, Marks JR, Berchuck A. Trinucleotide repeat polymorphisms in the androgen receptor gene and risk of ovarian cancer. Cancer Epidemiol. Biomarkers. Prev. 16: 473-480, 2007.
26. Weidman, J.R., Dolinoy, D.C., Murphy SK, and Jirtle, R.L. Cancer susceptibility: epigenetic manifestation of environmental exposures. Cancer J. 13: 9-16, 2007.
27. Hempel N, How T, Dong M, Murphy SK, Fields TA, Blobe GC. Loss of betaglycan expression in ovarian cancer: role in motility and invasion. Cancer Res. 67: 5231-5238, 2007.
28. Baba T, Mori S, Matsumura N, Kariya M, Murphy SK, Kondoh E, Kusakari T, Kuroda H, Mandai M, Higuchi T, Takakura K, Fukuda MN, Fujii S. Trophinin is a potent prognostic marker of ovarian cancer involved in platinum sensitivity.
Biochem. Biophys. Res. Commun. 360: 363-369, 2007.
29. O'Sullivan FM, Murphy SK, Simel LR, McCann A, Callanan JJ, Nolan CM. Imprinted expression of the canine IGF2R, in the absence of an anti-sense transcript or promoter methylation. Evol. Dev. 9: 579-589, 2007.
30. Baba T, Convery PA, Matsumura N, Whitaker RS, Kondoh E, Perry T, Huang Z, Bentley RC, Mori S, Fujii S, Marks JR, Berchuck A, Murphy SK. Epigenetic regulation of CD133 and tumorigenicity of CD133+ ovarian cancer cells. Oncogene: [Epub ahead of print], 2008